Faculty Profile: Shi, Xiaoyu

Return to Faculty Sponsors

Faculty Information Lab Information (Packet Type, Course Title, & Department) Location
Shi, Xiaoyu
Lab Contact:
Xiaoyu Shi
xiaoyu.shi@uci.edu
(949) 824-7638
A SUPER-RES IMAGING
Department: Developmental and Cell Biology
2403 Natural Sciences I

Research Description

Cells are the essential building blocks of life. They participate in the development and function of organs at all spatial scales – from molecular signaling to tissue patterning. The mission of this research program is to elucidate how cells drive developmental mechanisms across all scales. To answer these questions, we leverage super-resolution microscopy, live-cell imaging, chemical synthesis, and machine learning to develop novel cutting-edge imaging methods and apply these methods to study the following specific topics:

I. What is the structure of nuclear periphery ( nuclear lamina, nuclear pore complexes, nuclear envelope) at super resolution?
II. How does the nuclear periphery organize chromatin at molecular resolution?
III. How do primary cilia mediate cell signaling?

Methods: Expansion Microscopy (ExM), Stochastic Optical Reconstruction Microscopy (STORM), Live-Cell Sing-Particle Tracking, Structured Illumination Microscopy (SIM), Airyscan Microscopy, Confocal Microscopy, Total Internal Reflection Fluorescence Microscopy (TIRF), photoconversion

Requirements to Participate

1 year Commitment.

Faculty Means of Evaluation

Motivation: 10 Pts
Attendance: 10Pts (working assigned hours, being on time)
Lab Work: 50Pts (quality, accuracy, responsibility, and safety)
Communication: 20Pts (Written/Oral reports, questions and discussion with mentor)
Lab citizenship: 10Pts (organization, clean up and follow through)
Total: 100 points