Faculty Profile: Pearlman, Eric
|Faculty Information||Lab Information (Packet Type, Course Title, & Department)||Location|
949 824 1867
MICROBES & IMMUNITY
Department: Institute for Immunology
|Hewitt Hall 3032 (School of Medicine)|
My lab studies the host response to infections with pathogenic bacteria and fungi. Specifically, we examine the role of the innate immune cells neutrophils and inflammatory monocytes in immune regulation, microbial killing and in tissue damage. Specifically, we are studying the unique aspects neutrophil processing of inflammatory cytokines in the course of disease using human peripheral blood cells in addition to mouse models of disease. Bio199 students will work closely with graduate students and post-doctoral fellows in my lab to learn the molecular and cell biological techniques, and will read papers related to the projects.
Recent papers on fungal infections:
Sun, Y., S. Abbondante, M. Karmakar, S. de Jesus Carrion, C. Che, A. G. Hise and E. Pearlman. 2018. Neutrophil caspase-11 is required for cleavage of caspase-1 and secretion of IL-1ß in Aspergillus fumigatus infection. Journal of Immunology. 201(9):2767-2775. PMC6200591. (Featured in In this Issue as top 10% of papers)
Clark, H.L., S. Abbondante, M.S. Minns, Y. Sun, E. N. Greenberg, and E. Pearlman. 2018. Protein Deiminase 4 (PAD4) and CR3 regulate Aspergillus fumigatus and ß-glucan – induced neutrophil extracellular trap formation, but hyphal killing is dependent only on CR3. Frontiers Immunol. 9:1182. PMC5986955.
Clark, H. L., A. Jhingran, Y. Sun, C. Vareechon, S. de Jesus Carrion, E.P. Skaar, W.J. Chazin, J.A. Calera, T.M. Hohl, and E. Pearlman. (2016) Zinc and Manganese Chelation by Neutrophil S100A8/A9 (Calprotectin) Limits Extracellular Aspergillus fumigatus Hyphal Growth and Corneal Infection. J Immunol 196, 336-44. PMC4684987
Recent papers on bacterial infections
Vareechon C, S.E. Zmina, M. Karmakar, E. Pearlman, and A. Rietsch. (2017) Pseudomonas aeruginosa Effector ExoS Inhibits ROS Production in Human Neutrophils. Cell Host & Microbe 21: 611-618 e615. PMC5478421.
Karmakar, M., M. Katsnelson, G.R. Dubyak, and E. Pearlman. (2016) Neutrophil P2X7 receptors mediate NLRP3 inflammasome-dependent IL-1ß secretion in response to ATP. Nature Communications. 15; 7:10555. PMC4756306.
Karmakar, M., M. Katsnelson, N.G. Greene, H. A. Malak, Scott Howell, A. G. Hise, A. Camilli, A. Kadioglu, G. R. Dubyak and E. Pearlman. (2015) Pneumolysin induces K+ efflux and NLRP3/ Caspase 1 dependent IL-1ß processing by neutrophils. J. Immunol. 194:1763-75. PMC4369676.
Requirements to Participate
Having taken classes and have some laboratory experience in biochemistry, molecular biology or similar. 1 year Commitment.
Faculty Means of Evaluation
Attendance: 20Pts (working assigned hours, being on time)
Lab Work: 40Pts (quality, accuracy, integrated.synthesis of information and safety)
Communication: 20Pts (Written/Oral reports, questions and discussion with mentor)
Lab citizenship: 20Pts (organization, clean up and follow through)
Total: 100 points